![]() We anticipate that our LFIA will become a point-of-care test enabling rapid detection of CTX-M in hospital and community settings as well as a rapid environmental test.ĬTX-M ESBL LFIA Lateral flow immunoassay POCT Point-of-care test.Ĭopyright © 2021 Elsevier B.V. In conclusion, we developed a highly sensitive and specific LFIA capable of detecting CTX-M enzyme production in Enterobacterales. The rabbit monoclonal antibody did not cross-react with bacteria producing other class A β-lactamases, including SHV. The LFIA showed 100% sensitivity and specificity with clinical isolates on agar plates, and its limit of detection was 0.8 ng/mL recombinant CTX-M-14. This antibody detected CTX-M variants from the CTX-M-9, CTX-M-2, and CTX-M-1 groups expressed in clinical isolates. Here, we developed a lateral flow immunoassay (LFIA) based on an anti-CTX-M rabbit monoclonal antibody. CTX-M genes on plasmids have been transferred to many Enterobacterales species, and these species have spread, leading to the global problem of antimicrobial resistance. Electronic address: of CTX-M extended-spectrum β-lactamase-producing Enterobacterales are a severe threat in clinical settings. ![]() ![]() 7 Department of Microbiology and Immunology, Teikyo University School of Medicine, 2-11-1 Kaga, Itabashi-ku, Tokyo 173-8605, Japan.Electronic address: 2 Department of Microbiology and Immunology, Teikyo University School of Medicine, 2-11-1 Kaga, Itabashi-ku, Tokyo 173-8605, Japan Kyokuto Pharmaceutical Industrial Co., Ltd., 7-8 Nihonbashi Kobunacho, Chuo-ku, Tokyo, 103-0024, Japan. Electronic address: 1 Department of Microbiology and Immunology, Teikyo University School of Medicine, 2-11-1 Kaga, Itabashi-ku, Tokyo 173-8605, Japan. 6 Department of Laboratory Medicine, Teikyo University Hospital, 2-11-1 Kaga, Itabashi-ku, Tokyo 173-8605, Japan.5 Department of Microbiology and Immunology, Teikyo University School of Medicine, 2-11-1 Kaga, Itabashi-ku, Tokyo 173-8605, Japan.Tranquilize rabbits by injecting intramuscularly: acepromazine 0.75 mg/kg and butorphanol 0.2 mg/kg or dexmedetomidine 0.05 to 0.2. Allow a minimum of 7 days of acclimation after the arrival of the animals. 4 Abwiz Bio, Inc., 9823 Pacific Heights BLVD, Suite J, San Diego, CA, 92121, USA. Use young, adult, female Specific Pathogen Free rabbits (about 2 to 2.5 kg) whenever possible.3 Kyokuto Pharmaceutical Industrial Co., Ltd., 7-8 Nihonbashi Kobunacho, Chuo-ku, Tokyo, 103-0024, Japan.1 Department of Microbiology and Immunology, Teikyo University School of Medicine, 2-11-1 Kaga, Itabashi-ku, Tokyo 173-8605, Japan.
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